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KMID : 0644120080210020017
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Journal of Oriental Gynecology
2008 Volume.21 No. 2 p.17 ~ p.26
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Effects of Cervi Pontotrichum Cornu on Human Uterine Leiomyoma Cell in vitro
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Lee Yoon-Jae
Cho Jung-Hoon
Lee Jin-Moo
Jang Jun-Bock
Lee Kyung-Sub
Lee Chang-Hoon
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Abstract
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Purpose: This study was conducted to investigate the effects of Cervi Pontotrichum Cornu extract solution on the cell cycle regulation and apoptosis in human leiomyoma cell.
Methods: The leiomyoma cell of patients was used in the study, and we administered the extract solution of Cervi Pontotrichum Cornu concentration at 1, 10㎎/㎖ to the leiomyoma cell for 48 hours. We used flow cytometry and
western blotting to confirm cell cycle and apoptosis.
Results: In flow cytometry, G1 phase of the 1㎎/㎖ group prolonged. But G1 phase of 10㎎/㎖ group was shortened and S phase was increased. Cyclin D1 expression increased in higher concentration group. And Bax expression that regulates cell apoptosis increased in 1㎎/㎖ and 10㎎/㎖ group than control group. Bcl-2 expression decreased in 1, 10㎎/㎖ groups than control group. VEGF expression rised in higher Cervi Pontotrichum Cornu concentration group.
Conclusion: This study means that Cervi Pontotrichum Cornu could induce the apoptosis of leiomyoma cell by increasing Bax and decreasing Bcl-2 expression. But Cervi Pontotrichum Cornu could increase Cyclin D1 and VEGF
expression, so more detailed studies would be needed.
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KEYWORD
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Cervi Pontotrichum Cornu, leiomyoma, apoptosis, cell cycle
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